SRX14040020: PacBio HiFi whole genome sequencing of Anastrepha ludens: adult male thorax
1 PACBIO_SMRT (Sequel II) run: 4.8M spots, 41.3G bases, 20.3Gb downloads
Design: High molecular weight (HMW) DNA extraction was performed from the thorax using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in length to generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a 30 hour movie time on two SMRTcells. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.
Submitted by: United States Department of Agriculture
Study:
Genome sequencing of Anastrepha ludens adult male: principal pseudohaplotypeshow Abstracthide AbstractLong-reads were used to generate a whole genome assembly of Anastrepha ludens, the Mexican fruit fly
Sample:
Anastrepha ludens, Mexican fruit flyLibrary:
Name: SGAP062
Instrument: Sequel II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs:
1 run, 4.8M spots, 41.3G bases, 20.3Gb